Fig. 5. IFT20 and IFT88 localize to the base of the cilium in IFT57 mutant rods. (A) Wild-type rods expressing an IFT20-GFP transgene exhibit IFT20 localization at the base of the cilium (acetylated tubulin), where it colocalizes with acetylated tubulin (yellow; arrowhead). As the embryos do not carry an integrated transgene, only a small number of rod photoreceptors in the retina exhibit expression. (B) Loss of IFT57 does not affect IFT20-GFP localization to connecting cilia (arrowhead). Arrow indicates the connecting cilia in a neighboring cell that does not express the IFT20-GFP transgene. (C,D) In the presence or absence of IFT57, IFT20-GFP localized to the Golgi apparatus, where it colocalizes with syntaxin 6 (arrowhead). (E) IFT88 localizes to the base of the connecting cilium (arrowhead) in wild-type photoreceptors as well as in (F) IFT57 mutant photoreceptors. Abbreviations: AT, acetylated tubulin; Syn-6, syntaxin 6. Scale bar: 10 µm in A,B,E,F; 40 µm in C,D.