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Figure 7


Fig. 7. Yuri localization relative to {gamma}-tubulin and actin in controls. (A) {gamma}-tubulin staining positions the centriole/basal body at the center of the Yuri nuclear cap in round spermatids. (B) On elongating nuclei, the Yuri dot lies between the body of the nucleus and the CA, as identified by {gamma}-tubulin. (C) Diagram of the proposed location of Yuri on elongating nuclei. Adapted from Lindsley and Tokuyasu (Lindsley and Tokuyasu, 1980) with permission. (D) F-actin localization on round spermatid nuclei. (E-E'') Colocalization of Yuri and F-actin in the stripe and dot pattern seen on elongating nuclei. Arrow indicates actin/Yuri staining overlap on a single nucleus. (F-F'') Colocalization of actin and Yuri in moving actin cones. A cross-section of a set of large moving cones is shown. Yuri, green; nuclei, blue; {gamma}-tubulin, red in A-C; actin, red in D-F. Scale bars: 10 µm in A-D,E-E'', 20 µm in F-F''.