Fig. 1. Analysis of proteins in Tbdn immunoprecipitates. (A) Specificity of C10-20 antibody for Tbdn is demonstrated by immunoprecipitation (IP) of ³⁵S-labeled in-vitro-translated recombinant Tbdn protein with anti-Tbdn C10-20 antibody (Ab: Tbdn) or no antibody (Ab: –) in the absence of peptide (Peptide: –), or in the presence of control peptide (Peptide: Ctr) or Tbdn C10-20-blocking peptide (Peptide: Tbdn), followed by gel electrophoresis and Coomassie Blue staining (bottom) and autoradiography (top). Control ³⁵S-labeled in-vitro-translated Tbdn was also analyzed in parallel without immunoprecipitation (Lysate). (B) Immunoprecipitation of endothelial cell protein extracts using affinity purified anti-Tbdn antibody C10-20 (Tbdn IP) or no antibody (Control IP) followed by gel electrophoresis and silver staining. Bands of 69, 80 and 95 kDa are specific for Tbdn immunoprecipitates and are indicated by the arrows to the right of the gel.