Fig. 3. Colocalization of Tbdn with F-actin and cortactin in endothelial cells. (A-C,E-G) RF/6A endothelial cells grown on glass coverslips were stained with either phalloidin for F-actin (A,C; green fluorescence) or affinity purified anti-cortactin rabbit antibody (SC11408) (E,G; green fluorescence) and then double stained with OE5 monoclonal Tbdn antibody (B,C,F,G; red fluorescence). A merged image of A and B is shown in C, and a merge of E and F is shown in G (areas of colocalization: orange/yellow). Note areas of intense colocalized staining in perinuclear areas and intense colocalization at the cortex of motile cells. (D) Merged negative control stained with negative-control antibody and vehicle control. Representative confocal microscopy of slices of cells are shown. Scale bars: 20 µm.