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Figure 4


Fig. 4. Interphase MT disassembly and the formation of the mitotic spindle. Wild-type cells expressing GFP-atb2 and sid2-TOMATO (tubulin and SPB markers, respectively) progressing from interphase to mitosis were recorded in multiple focal planes. Maximum projections of eight z-series are shown. Arrowheads indicate the incipient formation of the mitotic spindle. Arrows indicate interphase MTs that are being disassembled as the intranuclear mitotic spindle is formed.