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Figure 1


Fig. 1. DN mutants of RhoG and ELMO inhibit cell spreading. (A) MEFs expressing GFP-RhoG-T17N, GFP-ELMOT625 or GFP alone were plated on 10 µg/ml fibronectin-coated coverslips in serum-free medium for 25 minutes, fixed and stained with Alexa-Fluor-568-conjugated phalloidin. Phalloidin staining of representative GFP-positive cells is shown. Scale bar: 10 µm. (B) Spread area of cells was calculated from 15 randomly selected GFP-positive cells for each condition. Values are means ± s.e.m.