Fig. 4. RhoG and Rac1 in cell migration. MEFs were transfected with pSuper, RhoG shRNA or Rac shRNA plus GFP (40:1 ratio). Time-lapse movies of randomly migrating cells were made as described in the Materials and Methods. Migration of at least 20 GFP-positive cells was analyzed from four independent experiments for each condition. Values are means for cell velocity (A) and directionality of migration (B) ± s.e.m. ^*P<0.001 (Student's t-test).