Fig. 5. Role of RhoG and Dock180 in Rac activation by integrins. (A,B) MEFs transfected with RhoG (A) or Dock180 (B) shRNA were replated on fibronectin and Rac activation measured as described in the Materials and Methods. Left panels: Rac pulldown assays. S, suspended cells; R, replated. Right panels: active Rac was quantified and normalized to total Rac. Values are means ± s.d.; n=3.^*P<0.05 (Student's t-test).