Fig. 7. RhoG-induced ruffling via Rac-dependent and -independent pathways. `Floxed' Rac1 MEFs were infected with Cre adenovirus and assayed after 6 days. (A) Representative immunoblot for Rac. (B) At 5 days after the infection with Cre, cells were transfected with GFP–RhoG-Q61L. After 24 hours, cells were fixed and stained for F-actin. (C) Ruffling area was quantified and is plotted against levels of GFP–RhoG-Q61L. Values are means ± s.e.m.; n>10 cells for each condition.