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Figure 7


Fig. 7. Localisation of PATJ and Lin-7 proteins in sdtK85-mutant adult photoreceptor cells upon expression of Sdt-encoding transgenes. Optical cross-sections of adult Drosophila eyes stained with anti-Sdt-N (A,B,C) or with anti-FLAG (D) (red), and with anti-PATJ (A,B) or anti-Lin-7 (C,D) (green). Phalloidin highlights the F-actin-rich rhabdomeres (blue). When Sdt-B2 is expressed in sdtK85 mutant PRCs (A-A"), both Sdt (A) and PATJ (A') localise at the stalk membrane. Upon Sdt-{Delta}PDZ expression in sdtK85 mutant PRCs (B-B"), Sdt (B) and PATJ (B') co-localise at the rhabdomere base. When Sdt-{Delta}L27C is expressed in sdtK85 mutant PRCs (C-C"), Sdt (C), but not Lin-7 (B') localises at the stalk membrane. When Sdt-{Delta}N is expressed in sdtK85 mutant PRCs (D-D"), Sdt (D) and Lin-7 (D') are distributed throughout the cell.