Fig. 8. Localisation of Crb in sdt^K85 mutant adult photoreceptor cells upon Sdt-encoding transgene expression. Optical cross-sections of adult Drosophila eyes stained with anti-Sdt-PDZ (red) and anti-Crb (green). Phalloidin highlights the F-actin-rich rhabdomeres (blue). (A) In sdt^K85-mutant eyes, which develop rhabdomeres with morphological defects, no Sdt (A) or Crb (A') was detected. (B) Sdt-B2 localises at the stalk membrane when expressed in sdt^K85-mutant PRCs (B) and restores localisation of Crb at the stalk membrane (B'). (C) Sdt-N expressed in sdt^K85 mutant PRCs is spread throughout the cells and is only occasionally slightly enriched near the stalk membrane (C), where it then colocalises with Crb (C', arrows). (D) Sdt-SH3 localises at the rhabdomere base when expressed in sdt^K85 mutant PRCs (D), and no Crb is detected (D').