Fig. 4. Further characterization of SVATs. (A) HeLa cells expressing GFP-SNX1 were infected with SL1344, fixed after a 15-minute infection and treated with TRITC-phalloidin and DAPI. GFP-SNX1 (green) creates a meshwork around bacteria (blue). Images of a deconvolved stack are shown (see supplementary material Movie 3). (B,C) Tubulation is not an artifact of overexpressing GFP-SNX1. Cells infected for 15 minutes were fixed using 4% paraformaldehyde at 37°C for 15 minutes and immunolabeled for endogenous SNX1 (Alexa488, green). Scale bar: 10 µm.