Fig. 7. In SNX1-suppressed cells, bacterial progress is slowed. (A,B) Analysis of intracellular progress of wild-type and sigD bacteria in control and SNX1-suppressed cells. After 15 minutes, non-internalised bacteria were removed from siRNA-treated cells. Cells were subsequently fixed after a total of 180 minutes of incubation. After appropriate immunolabeling, cells were imaged and the distance covered by bacteria from the plasma membrane to the nucleus was measured for individual bacteria and expressed as a percentage of distance covered (at least 100-200 bacteria were measured). (C) Graphical representation of distance of bacteria from nucleus for wild-type and sigD bacteria in control or SNX1 suppressed cells (± s.d). All P-values were calculated as comparisons with the wild-type value using a one-way ANOVA, followed by a post-hoc test (see Materials and Methods); ^**P<0.01.