Fig. 7. The assessment of the formation of plectin 1 complexes with F-actin, -dystrobrevin and β-synemin by blot overlay assays. PleN1 fragments (1 µg) immobilized on nitrocellulose were incubated with 3 µM F-actin (A) or 1 µM -dystrobrevin fragments (D) or 1 µM GST-fused full-length β-synemin (S) or the mixture of the three proteins (M), followed by detection of bound proteins using antibody specific for each protein. The signals resulting from the overlay with the three proteins were almost equivalent to those from the overlay with each protein alone. Values represent the mean ± s.d. (error bars) of two spots in three independent experiments.