Fig. 2. OxLDL colocalisation with LOX-1. (A) HeLa cells transfected with LOX-1-FLAG were incubated with labelled OxLDL (red) and mouse anti-FLAG antibodies on ice (1 hour) and then warmed to 37°C for the indicated times before fixation. Cells were permeabilised and antibodies bound to LOX-1 detected using FITC-conjugated anti-mouse IgG antibodies (green). Boxes display enlarged areas with colocalisation appearing yellow. Scale bar: 10 µm. (B) Amount of colocalisation of labelled OxLDL and LOX-1 was calculated (n=3 separate experiments in each of which five cells were quantified, values are mean ± s.e.m.). The P values calculated compared with values at t=0 minutes are indicated by asterisks, ^*P<0.05; ^**P<0.01. Values were expressed as a percentage of the colocalisation at t=0 minutes.