Fig. 4. Immunostaining of wild-type, hop2A and spo11 meiotic MICs. (A-C) Cna1 staining of centromeres demonstrates that, starting from a clustered arrangement and passing through a stage of dispersal, centromeres occupy a small area in the tip of the elongated MIC in wild type and in the hop2A mutant. In the spo11 mutant they remain dispersed. The DSB marker -H2A.X delineates MICs in wild type (D) and in the hop2A mutant (E), and in the spo11 mutant only after DSB induction by cisplatin (F). The recombination marker Rad51p forms foci in the elongating MICs of wild type (G) and the hop2A mutant (H), and displays a uniform nuclear distribution at later stages. It does not form foci in the spo11 mutant (I). Scale bar: 10 µm.