Fig. 1. Translocation of Bax from cytosol to mitochondria was correlated with Cyt c release during UV-induced apoptosis. (A) Western blots showing that Bax translocation was temporally correlated with Cyt c release, caspase-3 activation and PARP cleavage in HeLa cells during apoptosis. Cytosolic (C) and membrane (M) fractions of HeLa cells were harvested at different time points following UV irradiation. The bands marked ^* were the loading controls. (B) Quantitative analysis of the western blot results as shown in A. (C) Western blot analysis comparing the changes of the cytosolic fractions of GFP-Bax and endogenous Bax in HeLa cells following UV irradiation. (D) Quantitative analysis of the western blot results as shown in C.