JCS -- Gan et al. 121 (13): 2235 Figure IG7

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Fig. 7. The expression level of PPAR ${gamma}$ . (A) The relative amounts of p16, PPAR ${gamma}$ and GAPDH mRNA in young (Y; PD25), middle-aged (M; PD42) and senescent (S; PD62) 2BS cells. Total RNA was isolated as indicated and then subjected to RT-PCR analysis using PPAR ${gamma}$ or p16 primers. GAPDH was used as an internal control for normalization purposes. (B) Western blot analysis of PPAR ${gamma}$ and p16^INK4 expression in 2BS and WI-38 cells. Total proteins were extracted, and western blotting was performed using specific antibodies against PPAR ${gamma}$ and p16^INK4 as indicated. The β-actin lane serves as a loading control.