Fig. 6. H₂O₂ induces Src-kinase activation. C2 myoblasts were incubated with 10 µm H₂O₂ for 15 minutes. Immunoblots were probed for pY418-Src, striped then re-probed for total Src. A sample blot is shown. The graph represents the fold change in Src activation compared with control (pY418-Src/total Src). H₂O₂ incubation increased Src phosphorylation/activation 1.8 times compared with control cells. Such an increase was completely blocked when cells were incubated with PP2 prior to H₂O₂. Graph represents mean ± s.e.m. of four dishes per group from two independent experiments. ^*P<0.05; ^***P<0.001.