Fig. 5. (A) Increase in EGFR internalization in MDCK cells expressing CD9, TGF or CD9 and TGF. Cells were cultured in Transwell chambers, thus allowing full polarization, fixed and immunostained for EGFR. Immunofluorescence was visualized using confocal microscopy (xy section). (B) Cells were grown in regular culture medium, lysed, immunoprecipitated with anti-EGFR antibody and immunoblotted with antibodies against EGFR phosphorylated at tyrosine, EGFR or tubulin to detect phosphorylated EGFR (P-EGFR), total EGFR or tubulin.