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Figure 7


Fig. 7. Coexpression of CD9 and TGF{alpha} alters cell migration and adhesion. (A) Migration of MDCK cells expressing CD9, TGF{alpha} or both was analyzed in a Boyden chamber assay. Cell migration towards medium with 10% serum was quantified after 24 hours by counting those cells that had migrated through the membrane. Numbers of migrating cells were plotted in percent; 100% migration was defined as the number of migrated wild-type cells. (B) Cell adhesion was examined by Crystal Violet staining of the adherent cells after 20 or 40 minutes after seeding onto fibronectin-coated wells.