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Figure 3


Fig. 3. FCPT redistributed TPX2 and {gamma}-tubulin from the spindle pole of bipolar spindles. (A-D) Fixed images of spindles treated with (and without) FCPT for approximately 20 minutes: tubulin (red), TPX2 (A; green), {gamma}-tubulin (B; green), MCAK (C; green), XCTK2 (D). (A,B) FCPT redistributed the localizations of TPX2 and {gamma}-tubulin from the spindle pole to the equator of the mid-spindle. (C,D) FCPT removed the localization of MCAK and XCTK2 from the spindle pole. (E) Fixed images of FCPT-treated DMSO asters after (FCPT post-assembly) and before (FCPT pre-assembly) aster assembly: tubulin (red) and TPX2 (green). FCPT addition after DMSO aster formation (FCPT post-assembly) had no effect on the aster pole and localization of TPX2, whereas FCPT treatment before aster formation (FCPT pre-assembly) perturbed aster pole assembly and the pole localization of TPX2. Scale bars: 10 µm.