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Figure 1


Fig. 1. Expression pattern of TRPCs in hippocampal development. (A) Western blot analysis of rat hippocampi at the indicated ages with the indicated antibodies. Ad, adult. Positions of molecular size markers (in kDa) are indicated on right. (B) In situ hybridization of P14 brain sections shows that TRPC6 is expressed in all regions of the hippocampus. (C) Immunohistochemical analysis of rat hippocampi at the indicated ages with TRPC6 antibody. (D) Confocal laser-scanning microscopy images of TRPC6 (green) and MAP2 (red) immunolabeling of P14 hippocampal CA1 neurons. Scale bar: upper left, 50 µm; enlargements of boxed area, 10 µm.