Fig. 4. The Ca²⁺ influx through TRPC6 is important for dendritic development. (A,B) Quantification of total dendritic tips and total dendritic length of hippocampal neurons transfected with the indicated constructs at 5 DIV for 2 days. Vehicle, 2 mM EGTA or 10 µM Cd²⁺ was applied for 24 hours before neurons were fixed. Data are the mean ± s.e.m. n.s., not significant. (C) Effect of downregulation of TRPC6 on OAG-induced [Ca²⁺]_i elevation. Intracellular Ca²⁺ levels in the neurons transfected with pPRIME-FF3 or shTRPC6 RNAi were determined by the F340/F380 ratio. The relative change in [Ca²⁺]i was depicted by 340/380 ratio normalized to the baseline. Data are mean ± s.e.m. of 45 cells from three independent experiments. (D,E) Quantification of total dendritic tips (D) and total dendritic length (E) of the neurons transfected with the indicated constructs at 3 DIV for 4 days. DMSO or 100 mM OAG was applied for 24 hours before the neurons were fixed. ^**P<0.01 versus control.