Fig. 5. TRPC6-induced dendritic growth depends on CaMKIV activity. (A) Total lysates of cells treated as indicated blotted with antibodies to phosphorylated CaMKIV, total CaMKIV, phosphorylated CaMKII or total CaMKII. (B) Quantification of CaMKIV phosphorylation in the neurons transfected with the indicated constructs. ^*P<0.05 versus ctrl. (C) Representative images of the neurons transfected at 3 DIV for 4 days with wild-type TRPC6 (WTC6) and the dominant-negative CaMKIV mutant, or shTRPC6 RNAi construct and the constitutive active CaMKIV mutant. Scale bar: 20 µm. (D,E) Quantification of total dendritic tips (D) and total dendritic length (E) of the neurons shown in C. ^**P<0.01 versus ctrl. All data are the means ± s.e.m. n.s., not significant.