Fig. 2. UbcH10 levels increase at a similar time to cyclin A in late G1 phase. (A) HeLa cells were released from a dimethylenastron-induced prometaphase block and samples taken at the indicated times (hrs). Samples were analysed by propidium iodide staining and flow cytometry (top) and by immunoblotting with the indicated antibodies (bottom). Results are representative of two independent experiments. (B) Serum-starved hTert-RPE cells were released into the cell cycle by adding 20% serum and harvested at the indicated time points (hrs). Samples were analysed by propidium iodide staining and flow cytometry (bottom) and by immunoblotting with the indicated antibodies (top). Results are representative of five independent experiments.