Fig. 2. Activators or inhibitors of cPKC and nPKC isoforms do not affect PCTV-budding activity. The effect of PMA and Ro-31-8220 on PCTV-budding activity. (A) PCTVs were allowed to bud from native intestinal ER using native cytosol (NC) or ER treated with PMA (100 nM). PCTV-budding activity is shown. (B) PCTV-budding activity using native cytosol (Native cytosol) or cytosol treated with 50 nM Ro-31-8220 (Ro-31-8220). (C) The PKC isoform is important for PCTV-budding activity as suggested by the concentration of the PKC inhibitor Gö 6983 that is required to inhibit budding. PCTV-budding activity using native cytosol (NC) or cytosol treated with the indicated amount of Gö 6983. Data are expressed as a percentage of dpm of [³H]TAG in PCTV budded from the ER using native cytosol as 100%. P values indicate differences between the means, or between 60 nM Gö 6983 and either native cytosol or cytosol treated with 10 nM Gö 6983. Data are the mean ± s.e.m., n=4.