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Figure 4


Fig. 4. Successful removal of PKC{zeta} from cytosol and ER. (A) Cytosol was twice treated either with bead bound IgG (Cyto) or bead bound anti-PKC{zeta} antibodies (PKC{zeta}-dep Cyto). 30 µg of treated cytosol protein were separated by 12% SDS-PAGE, transferred to a nitrocellulose membrane and PKC{zeta} identified by immunoblot (ECL). (B) ER was treated either with cold HEPES (ER) or cold HEPES plus 2 M urea (2M Urea washed ER). The proteins were separated by 12% SDS-PAGE. PKC{zeta} was detected by immunoblotting (ECL).