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Figure 6


Fig. 6. Pre-phosphorylating intestinal ER with rPKC{zeta} and ATP supports PCTV budding. Bar 1 describes the results obtained when [3H]TAG pre-loaded intestinal ER (500 µg) and cytosol (1 mg) were incubated with ATP and PCTV-budding activity was determined. Bar 2 reports the PCTV-budding activity obtained when pre-phosphorylated and [3H]TAG pre-loaded ER (500 µg) (Materials and Methods) were incubated with PKC{zeta}-depleted cytosol (1 mg). Bar 3 shows the PCTV-budding activity obtained when pre-phosphorylated and [3H]TAG pre-loaded ER (500 µg) was incubated with recombinant L-FABP (40 µg).