Fig. 5. SCA14 mutations located in C1B open PKC protein conformation. (A) Fluorescence lifetime imaging analysis of the indicated constructs, showing in each column, from left to right, the fluorescence intensity, false-color map of the fluorescence lifetime calculated from the phase shift (tau-phi) and histogram of the lifetime distribution with the same false-color scale as the lifetime map. Scale bar: 10 µm. (B) The table summarizes the average phase and modulation lifetimes (± s.d.). The number of cells measured is indicated by n. (C) FRET efficiency (mean ± s.e.m.; unpaired t-test, ^***P<0.001) and quantification are calculated based on the phase lifetimes, using the SCFP3A lifetime as the control.