Fig. 3. Localization of GFP-RhoA in control and in nocodazole-treated cells. Fluorescence images of living LLC-Pk1 cells transfected with GFP-tagged C. elegans RhoA (GFP-CeRhoA). In control cells (A) and nocodazole-treated cells that assemble a contractile ring (C), GFP-CeRhoA localizes to the equatorial cortex during cytokinesis. In nocodazole-treated cells that fail to assemble a contractile ring (B), GFP-CeRhoA fails to accumulate at the equatorial region; asterisks mark the location of chromosomes. In both control and nocodazole-treated cells, GFP-CeRhoA also localizes to membranous folds between neighboring cells. Time is shown in minutes:seconds and is relative to the addition of nocodazole in B and C. Scale bars: 10 µm.