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Figure 8


Fig. 8. Blocking of {alpha}5 integrin partially inhibits fibronectin endocytosis. FN-null myofibroblasts were incubated with 50 µg/ml {alpha}5 inhibitory antibodies (5H10-27) or 100 µg/ml {alpha}v inhibitory antibodies (H9.2B8) or isotype control IgG at 4°C for 30 minutes. 10 µg/ml TR-fibronectin (A-D) or AF488-fibronectin (E) was added to the medium. After 2 hours of chase, cells were either fixed for imaging analysis (A, {alpha}5 inhibition; B, isotype control of {alpha}5; C, {alpha}v inhibition; D, isotype control for {alpha}v), or processed for flow cytometry (E) to quantify internalized fibronectin. The numbers over the peaks in E are the MFI of internalized AF488-fibronectin. Scale bar: 10 µm.