Fig. 2. Twisted growth of spr2 is enhanced in spr2sp2l. (A) RT-PCR analysis of SPR2 and SP2L expression in spr2 and spr2sp2l mutant alleles. See Fig. 1A,B for experimental conditions. (B) Twisted petioles in 9-day-old seedlings of spr2 and spr2sp2l. (C) Rotated angles of primary leaves in 2-week-old seedlings. The number of leaves analyzed is shown in the upper right corner. (D) Inflorescence stem of spr2-2sp2l-1 climbs up a support, forming a right-handed helix. Three complete turns are shown by red arrows. (E) Root growth of 7-day-old seedlings grown on vertically placed hard agar plates. (F) Quantitative analysis of the root slanting angles. Roots that grew toward the right side of the plates were assigned the plus sign, whereas roots that grew to the opposite direction had the minus sign in the root-slanting angles. Error bars represent standard deviations of the means. (G) Overexpression of SP2L under the control of the CaMV35S promoter rescued the twisting phenotype of spr2-1. (H) SPR2-GFP expressed in the genomic context rescued the twisting phenotype of spr2-2. Nine-day-old seedlings are shown in G and H.