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Figure 1


Fig. 1. Characterization of the eIF3k antiserum. (A) Western blot analyses on lysates from untransfected HeLa cells or HeLa cells transfected with FLAG-tagged eIF3k (eIF3k-Flag), or on GST-eIF3k purified from bacteria (GST-eIF3k). Antibodies used are eIF3k antiserum, anti-Flag and eIF3k antiserum pre-absorbed with GST-eIF3k. (B) Immunoprecipitation analysis with the eIF3k antiserum or a control antiserum. Lysates of HEK 293T cells were used for immunoprecipitations with antibodies as indicated, followed by western blot with eIF3k antiserum. (C) Expression of eIF3k in various cells as indicated. All cell lines are from human origin except for NIH3T3 (3T3) and Cos-1 (Cos).