Fig. 8. GFP-AFAP-110S277A expression increases the average lifespan of a podosome. (A) Percentages of podosomes were plotted against their lifespan in incremental 10-minute time brackets. The mean percentages of podosomes observed in cells from three different experiments is given ± s.d. (B) The average lifespan of podosomes was 17.9±17 minutes in cells that express GFP-AFAP 110 wild type (gray bar) and 28.6±21 minutes in cells that overexpress GFP-AFAP-110S277A (black bar); the difference is statistically significant (P<0.0001).