Fig. 6. ApoB in the ApoB-crescent tightly adheres to the LD surface through non-ionic interactions. (A) Gallery of isolated LDs labeled for ApoB (green; goat polyclonal) and ADRP (red). LDs were isolated from Huh7 cells treated with 0.4 mM DHA for 12 hours. ApoB and ADRP were observed in complementary areas of LDs. Bar, 5 µm. (B) LDs isolated from DHA-treated cells were incubated with 0.1 M sodium carbonate for 30 minutes at 4°C, and fractionated by the second round of density-gradient centrifugation. Using western blotting, most ApoB and ADRP were recovered from the top floating LD fraction, whereas cyclophilin B was shifted to the bottom soluble fraction.