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Figure 2


Fig. 2. Aconitase-MTS point mutations. (A) Growth and complementation of aconitase-MTS point mutants. {Delta}aco1 strain harboring the indicated plasmids encoding MTS point mutants were diluted and grown on galactose or ethanol-acetate medium plates as indicated. (B) Aconitase-MTS point mutants are processed. MTS point mutants of aconitase were labeled and examined for processing as described in the legend to Fig. 1B. (C) MTS point mutants exhibit alterations in the protein subcellular distribution. Yeast cells expressing aconitase variants were subjected to subcellular fractionation. Equivalent portions from the total (Tot) cytosolic (Cyt) and mitochondrial (Mit) fractions were analyzed by western blotting using antibodies against the indicated proteins.