Fig. 6. (A) Translocation machinery mutant strains exhibit alterations in protein subcellular distribution. Indicated mutant strains were grown at the permissive temperature. The strains were subjected to subcellular fractionation. Equivalent portions from the total (Tot) cytosolic (Cyt) and mitochondrial (Mit) fractions were analyzed by western blotting using antibodies against the indicated proteins. (B) Growth at low temperature increases the aconitase cytosolic presence. Wild-type yeast cells were grown at 20°C or at 30°C and subjected to subcellular fractionation as described above.