Fig. 7. Translocation of aconitase-MTS mutants is slower. Yeast cells expressing the indicated plasmids were incubated for 1 minute with CCCP followed by labeling for 15 minutes with [³⁵S]methionine (in the presence of CCCP). DTT, an excess unlabeled methionine-cysteine and cycloheximide were added (start of the chase). Aliquots were taken at the indicated times, immunoprecipitated with aconitase antiserum and analyzed by SDS-PAGE. Arrows show positions of precursor (p) and mature proteins (m).