Fig. 1. Generation of Tex12–/– mice. (A) Exon 2 to exon 5 of the Tex12 gene were replaced by a neomycin cassette to generate Tex12–/– mice. (B,C) The mice were genotyped using PCR (black primers in A, two in the gene, one in the neomycin cassette) and did not contain the Tex12 RNA (RT-PCR, grey primers in A, positive control using Trip13) or protein (western blot, WB, positive control
-tubulin).