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Figure 3


Fig. 3 The Lsm4 C-terminus is required for efficient localization of Lsm1p to P-bodies. (A) GFP-Lsm1 (pGFP-N-Lsm1) localization 20 minutes after hypo-osmotic shock in LSM4 (MRY71) or lsm4{Delta}C (MRY73) cells. (B) Percentage of cells showing GFP-Lsm1 in foci 5 minutes or 1 hour after osmotic shock (n=100 cells per time point). (C,D) Localization of GFP-Lsm2 (pMPSLsm2) (C) and GFP-Lsm6 (pMPSLsm6) (D) in LSM4 or lsm4{Delta}C cells before and after hypo-osmotic shock (E) Dcp2-RFP (pMR159) localization in log phase LSM4 and lsm4{Delta}C cells grown in SD-His and 20 minutes after osmotic shock. All experiments in this figure were performed with strains expressing non-tagged Lsm4p or Lsm4{Delta}Cp from the native LSM4 promoter.