Fig. 1. cfaD^– cells lack cfaD mRNA and CfaD protein. (A) Northern blot of RNA from wild type (WT) and cfaD^– vegetative cells probed for cfaD. A loading-control gel stained with ethidium bromide showed apparently equal quantities and lack of degradation of the ribosomal RNA bands. (B) Western blot of total cell lysates from mid-log vegetative cells stained with affinity-purified anti-CfaD antibodies. Molecular weight markers (in kDa) are given at the right. A loading-control gel stained with Coomassie Blue showed apparently equal quantities of proteins in all samples. (C) Disruption of cfaD affects the appearance of fruiting bodies. Cells of the indicated strain were grown on bacterial lawns and fruiting bodies were photographed. Bar, 0.5 mm.