Fig. 5. CfaD appears to interact with AprA. rHMCfaD and nickel-agarose beads were mixed overnight with conditioned growth medium (CGM) prepared from wild-type and aprA^– cells. After washing with PBS, proteins bound to the beads were eluted with SDS sample buffer. Western blots of the bound proteins were stained with anti-Myc antibodies (top left panel) or anti-AprA antibodies (lower left panel). Similarly, rAprA was mixed with nickel-agarose beads and CGM from wild-type and cfaD^– cells; western blots of the bound material were stained with anti-Myc antibodies (top right panel) or anti-CfaD antibodies (lower right panel). Molecular mass markers in kDa are shown in the middle.