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Figure 5


Fig. 5. The laminin composition of KO/Tg small intestine subepithelial BMs resembles that of the normal colon. Intestine sections were stained with antiserum directed against laminin {alpha}-chains, as indicated. (A) Laminin {alpha}5 was detected in the control subepithelial BM of villi but not of crypts. (B) Laminin {alpha}5 staining was greatly reduced in villus BM (arrow) of KO/Tg mice but was detectable in both the mesenchymal structures within villi and the intestinal smooth muscle wall. (C) Low levels of laminin {alpha}1 were detected in the control subepithelial BMs of both villi and crypts. (D) Levels of {alpha}1 were increased in KO/Tg villus BM. (E) In controls, high levels of laminin {alpha}4 were detected in the endothelial BM of blood vessels, but not in the villus subepithelial BM (arrowhead in inset). (F) In KO/Tg, in addition to endothelial BM, laminin {alpha}4 was deposited in the villus subepithelial BM (arrowhead in inset). (G-L) Compared with WT distal small intestine, laminin {alpha}5 was only weakly deposited in the subepithelial BMs of WT colon (arrow in H), whereas laminins {alpha}1 and {alpha}4 were abundant. Arrowheads in the insets of K and L point to the position of epithelial BMs. Dashed horizontal lines indicate the crypt-villus boundary. Bars, 100 µm.