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Figure 1


Fig. 1. Structure of Nok deletion mutants and their expression. (A) Nok is a MAGUK protein which contains ECR1, a bipartite L27 domain, PDZ, SH3, 4.1 and GUK domains. Overview of different Nok deletions functionally tested in this study. Asterisk indicates the position of the nokm520 mutation. Expected interactions (black arrows) between Nok with different members of the Crb and the Par6-aPKC protein complexes are indicated. (B) Western blot of 32 hpf tissues with Myc-tagged Nokwt (asterisk; expected size of 82 kDa), Nok{Delta}Crb (asterisk; expected size of 74.5 kDa), Nok{Delta}C (dot; expected size of 50.6 kDa), Nok{Delta}Par6 (asterisk; expected size of 81.1 kDa), Nok{Delta}Patj (asterisk; expected size of 75.7 kDa), and Nok{Delta}Patj{Delta}Lin7 (asterisk; expected size of 69.4 kDa). Loading control is acetylated tubulin (bottom).