Fig. 3. Cardiac development in Nok deletion mutants. (A-F) Images represent reconstructions of confocal Z-stack sections of 32 hpf Tg[cmlc2:GFP] transgenic and nok morphant embryos (coinjected with MO^p53 to suppress MO^nok off-target effects) expressing different Nok deletion proteins. Cardiac morphology is assessed using the atrium-specific antibody S46 (red). Arrows indicate the extent of atrial expansion. (C) Whereas nok^wt or (E) nok^PatjLin7 mRNA complements nok morphant heart defects, all other Nok deletions lack rescue function in this assay. The corresponding mutant cardiac phenotypes are characterized by an arrest at the heart cone stage or by a failure to extend the heart tube. (G) Summary of rescue efficiencies of the different Nok deletions expressed within the nok morphant background.