JCS -- Bit-Avragim et al. 121 (15): 2503 Figure IG6

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Fig. 6. Apical localization of Pard6 ${gamma}$ b within the neural tube does not require the PDZ domain. (A-C) Live 24 hpf embryos injected with H2b-mRFP and pard6 ${gamma}$ b^wt-GFP or pard6 ${gamma}$ b^KPLG-GFP mRNA as indicated (dorsal views of the neurocoel between the first and sixth somite). (A,B) In WT, both pard6 ${gamma}$ b^wt-GFP and pard6 ${gamma}$ b^KPLG-GFP localize along the apical ventricular surface of the neural tube (white arrowheads). (C) Within the pard6 ${gamma}$ b^s441 mutant background, pard6 ${gamma}$ b^KPLG-GFP localizes correctly to apical surfaces of the neural tube suggesting alternative modes of apical localization of pard6 ${gamma}$ b independent of the PDZ-domain-mediated association with Crb or Nok. (D-F) Localization of pard6 ${gamma}$ b^wt-GFP within the neural tube is dependent on either (E) Nok or (F) Ome/Crb2a. (H) Apical localization of Nok requires Ome/Crb2a. Orientation: all images are apical views.