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Figure 2


Fig. 2. FRAP analysis of core-localizing NE proteins. (A) HeLa cells stably expressing core-localizing NE proteins were examined by FRAP analysis. Images of GFP-BAF are shown as an example. The bleached region is the 2 µm region indicated by the white square. (B-D) FRAP profiles of GFP-BAF (B), GFP-lamin A (C) and GFP-emerin (D). Closed circles, fluorescence intensity in the bleached region; open circles, fluorescence intensity in the unbleached region. Bars represent s.d. Number of cells examined was 7 for GFP-BAF, 5 for GFP-lamin A and 5 for GFP-emerin.