Fig. 8. Live-correlated EM analyses of HeLa cells after siRNA treatment for BAF. (A) Time-lapse images for chromosomes and GFP-BAF were taken for control cells. Cells were fixed at 12 minutes after the metaphase-anaphase transition. Only images for GFP-BAF are shown. See supplementary material Fig. S4 for more details. (B) An EM image of the same cells shown in A. (C) A fluorescence micrograph of the same cells shown in A: selected focus from a series of 3D images. Magenta represents chromosomes (Hoechst 33342) and green represents GFP-BAF. (D) Magnified view of the region indicated in the larger square in B. Drawings are superimposed on the EM images indicating the NE (red) and MTs (orange). (E) High-magnification EM images of the small box indicated in B. (F) Time-lapse images for chromosomes and GFP-BAF were taken for BAF RNAi cells. Cells were fixed at 12 minutes after the metaphase-anaphase transition. Only images for GFP-BAF are shown. See supplementary material Fig. S4 for more details. (G) EM images of the same cells shown in F. (H) A fluorescence micrograph of the same cells in F: a selected focus from a series of 3D images. Magenta represents chromosomes (Hoechst 33342) and green represents GFP-BAF. (I) Magnified view of the region indicated in the largest square in G. (J) Drawings are superimposed on the EM images of I, indicating the NE (red) and MTs (orange). (K,L) High-magnification EM images of the small boxes indicated in G: K, left box; L, right box. (M) An EM image of another example of BAF siRNA-treated cells. (N) A fluorescence micrograph of the cells shown in M. Magenta represents chromosomes (Hoechst 33342) and green represents GFP-BAF. (O) Enlarged image of the boxed region in N and corresponding to the boxed region in M. (P) Magnified view of the boxed region in M and corresponding to the image shown in O. (Q) Drawings are superimposed on the EM images of P, indicating the NE (red) and chromosomes (magenta). Scale bars: 10 µm (A,F), 2 µm (B,C,G,H,M,N), 500 nm (D,J,P,Q) and 200 nm (L).