Fig. 1. (A-F) Axonal outgrowth from freshly dissected (A,C,E) and in-vitro conditioned (B,D,F) PN-DRG. In vitro conditioned PN-DRG show enhanced BDNF-induced axonal outgrowth in the presence or absence of ActD. After 3 days in collagen gels, freshly dissected PN-DRG show limited axonal growth (A) that is markedly increased by BDNF (C) but not in the presence of ActD (E). In vitro conditioned preparations show slightly increased axonal outgrowth (B) that is greatly enhanced by BDNF (D), even in the presence of ActD (F). Note that not all axons are within the plane of focus. Scale bar: 200 µm.